目的 为菌根真菌在铁皮石斛栽培生产中的应用奠定基础。方法 以生物量为指标,在种植基地筛选出优势菌株;进一步考察其初始用菌量和补充施用对铁皮石斛生长和化学成分的影响。结果 栽培16个月,接种优势菌株M2(Mycena sp.)的3个处理组茎干重均显著高于对照组(P<0.05);其中初始用量1.0 g·丛-1的处理(M)茎干重最高,比对照提高了39.2%,比初始用量0.5 g·丛-1的处理(L)提高了11.1%(P<0.05);菌根栽培对铁皮石斛茎多糖化学性质基本没有影响,能提高茎中柚皮素和联苄类化合物含量。菌根栽培后补充施菌,能增强M2促进植物生长的作用,补充施菌组茎干重比未补充施菌组提高了18.0%(P<0.05),但补充施菌对多糖化学性质有一定影响。结论 菌株M2用于铁皮石斛的栽培生产有良好的应用前景。
Abstract
OBJECTIVE To provide the scientific basis for the application of mycorrhizal fungi in Dendrobium officinale artificial cultivation. METHODS The dominant strain was screened in a planting base by using biomass as the index. The effects of initial inoculums and supplementary inoculation on the growth and the chemical composition of D. officinale were further investigated. RESULTS Mycorrhizal fungi M2(Mycena sp.) was found to be the advantageous strain. After 16 months of growth, the stem dry weight(SDW) and plant dry weight(PDW) of the three M2-treatment groups were significantly higher than those of CK, and the SDW of group M(M2 initial inoculums of 1.0 g·clump-1) was the highest, which increased by 39.2% and 11.1% compared with CK and group L(M2 initial inoculums of 1.0 g·clump-1)(P<0.05), respectively. Furthermore, M2 had no effect on the chemical properties of stem polysaccharide, and could improve the contents of naringenin and bibenzyls in the stems. The supplementary inoculation of M2 enhanced the plant-growth-promoting effects of M2. The SDW of the supplementary group increased by 18.0%(P<0.05) compared with the non-supplementary group, while supplementary inoculation had a certain effect on the chemical properties of polysaccharide. CONCLUSION Mycorrhizal fungus M2 has good application prospects for D. officinale artificial cultivation.
关键词
铁皮石斛 /
小菇属真菌 /
初始用菌量 /
补充施菌
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Key words
Dendrobium officinale /
Mycena sp. /
initial inoculums dosage /
supplementary inoculation
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中图分类号：
R284
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参考文献
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脚注
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基金
国家自然科学基金资助项目(81573594); 中医药行业科研专项资助(201407005)
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